BNIP3 Antibody (Rodent Specifi

3769:

Western Blotting

BNIP3 Antibody (Rodent Specific) detects endogenous levels of total BNIP3 protein from mouse and rat.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser60 of BNIP3 from mouse/rat. Antibodies were purified by peptide affinity chromatography.

Western Blotting

Western blot analysis of HeLa cells, mock transfected or transfected with mouse BNIP3, CTLL-2, RAW 264.7 and BaF3 cells using BNIP3 Antibody (Rodent Specific).

BNIP3 (Bcl-2/E1B-19kDa interacting protein 3) is a pro-apoptotic mitochondrial protein and Bcl-2 family member that contains a Bcl-2 homology 3 (BH3) domain and a carboxyl terminal transmembrane (TM) domain (1-3). While BNIP3 has a predicted molecular weight of about 22 kDa, it runs anomalously on SDS-PAGE and includes a band of around 60 kDa that may be a dimeric form that is not reduced (2). BNIP3 associates with anti-apoptotic family members Bcl-2, Bcl-xL, and the adenovirus homologue E1B-19kDa. BNIP3 is distinct from other Bcl-2 family members that contain only the BH3 domain in that the TM domain, and not the BH3 domain, is required for mitochondrial targeting and pro-apoptotic activity (4). In addition to apoptosis, BNIP3 has been implicated in necrosis (5) and autophagy (6,7), although the relationship between BNIP3 and these processes is not fully understood. Increased expression of BNIP3 under hypoxic conditions is mainly regulated by the transcription factor HIF-1α (8-10). Silencing of the BNIP3 promoter by methylation has been observed in several types of cancer cells and may play an important role in their survival (11-14).

1. Boyd, J.M. et al. (1994) Cell 79, 341-51.
2. Chen, G. et al. (1997) J Exp Med 186, 1975-83.
3. Yasuda, M. et al. (1998) J Biol Chem 273, 12415-21.
4. Ray, R. et al. (2000) J Biol Chem 275, 1439-48.
5. Vande Velde, C. et al. (2000) Mol Cell Biol 20, 5454-68.
6. Daido, S. et al. (2004) Cancer Res 64, 4286-93.
7. Tracy, K. et al. (2007) Mol Cell Biol 27, 6229-42.
8. Bruick, R.K. (2000) Proc Natl Acad Sci USA 97, 9082-7.
9. Guo, K. et al. (2001) Cell Death Differ 8, 367-76.
10. Sowter, H.M. et al. (2001) Cancer Res 61, 6669-73.
11. de Angelis, P.M. et al. (2004) Int J Oncol 24, 1279-88.
12. Okami, J. et al. (2004) Cancer Res 64, 5338-46.
13. Murai, M. et al. (2005) Clin Cancer Res 11, 1021-7.
14. Murai, M. et al. (2005) Br J Cancer 92, 1165-72.

• Shackelford, D.B. et al. (2009) Proc Natl Acad Sci U S A 106, 11137-42. Applications: Western Blotting

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For Research Use Only. Not For Use In Diagnostic Procedures.