- 询价
- Cell Signaling Technology
- 9548
- USA
- 2025年11月01日
- W
- M
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific)
- 抗原：
synthetic PARP peptide corresponding to carboxy-terminal residues surrounding Asp214 in mouse PARP
- 应用范围：
W
- 适应物种：
M
- 库存：
大量
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | M | Endogenous | 89 | Mouse IgG2b |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific) detects endogenous levels of the large fragment of mouse PARP1 (89 kDa) resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic PARP peptide corresponding to carboxy-terminal residues surrounding Asp214 in mouse PARP. Western Blotting
Western blot analysis of NIH/3T3 cells, untreated or staurosporine-treated (1 µM), using Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific). |
| Background | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). (This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Assessment of Insulin Secretion in the Mouse
from the cleavage site ( 4 ). In the rat, two separate 110-amino acid preproinsulins are transcribed from two nonallelic preproinsulin genes, from which two forms of insulin and C-peptide are subsequently cleaved ( 1 ) ( see Fig
Intracellular Cytokine Staining Protocol
that the investigators evaluate the use and efficacy of different protein transport inhibitors in their specific assay system.Note: Generally, the non-specific background staining of fixed and permeabilized cells is higher than surface staining; therefore, extra protein
，EDTA 缓冲液适用于多数磷酸化酪氨酸特异性抗体，而柠檬酸盐缓冲液适用于其他多数抗体。每天都需制备新鲜的 1X 溶液。抗体：hospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb #2842样本：蜡包埋人类肺部肿瘤抗原修复缓冲液：柠檬酸盐缓冲液（左）或者 EDTA 缓冲液（右）煮沸装置载玻片在所推荐的缓冲液中加热煮沸一段时间后，才可引起抗原修复。该步骤通常在微波炉或高压锅中进行。虽然，一些研究人员也使用水浴锅，但是，因为该步骤中所选择的装置对染色存在积极或消极的影响
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