- 询价
- Cell Signaling Technology
- 7561
- USA
- 2025年07月15日
- 免疫荧光（IF）,流式细胞（Flow Cyt）
- 兔
- 人,大鼠,驴,斑马鱼,细菌,猪
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
香烟烟雾如何加速声带纤维化？CD44-透明质酸轴是关键通路Takara 秋促来袭，提取+反转录+定量套装仅 1200 元五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
COX IV (3E11) Rabbit mAb (Alexa Fluor 647 Conjugate)
- 抗原：
/
- 应用范围：
免疫荧光（IF）,流式细胞（Flow Cyt）
- 宿主：
兔
- 库存：
大量
- 级别：
详见MSDS文件
- 抗原来源：
/
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
人,大鼠,驴,斑马鱼,细菌,猪
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Glucose Metabolism
COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) #7561
PhosphoSitePlus ® protein, site, and accession data: COX4I1
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC F | H R Mk Z B Pg | Endogenous | Rabbit IgG |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human R=Rat Mk=Monkey Z=Zebrafish B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 7561:
- Flow , Immunofluorescence
Specificity / Sensitivity
COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total COX IV protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV protein.
Flow Cytometry
Flow cytometric analysis of HeLa cells using COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) (green) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with Alexa Fluor® 488 phalloidin (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated COX IV (3E11) Rabbit mAb #4850.
Background
Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.
- Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
- Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
- Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
- Barrientos, A. et al. (2002) Gene 286, 53-63.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
For Research Use Only. Not For Use In Diagnostic Procedures.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验CST 抗体 9 月特惠｜购买任意 CST 抗体，+168 元即可入手 25μl 标签 / 内参抗体
HRP、Alexa Fluor® 488、Alexa Fluor® 594 等多种标记一抗可选。 热门可选产品参考 货号 产品名称 类型 加价购价格 S0B0005-25u1 B-actin Rabbit mAb (SDT-RO15) 裸抗 168 元 S0B0004-25u1 B-tubulin Recombinant Rabbit mAb (SDT-RO14) 裸抗 168 元 S0B0383-25u1 S-RMab® Myc tag Recombinant Rabbit
Antibody250µlIF-IC HCA4410SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4412SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F4413SAnti-rabbit IgG (H+L
IF/mIHC/空间组学切片选型指南：选对冰冻、FFPE少走弯路
(SDT-132-63) 一抗 S0B2039 MCM3 Recombinant Rabbit mAb (SDT-048-10) 一抗 S0B4004 Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 488 Conjugate) 二抗 二、多重染色mIHC 多重染色的瓶颈不是"每种抗体好不好染"，而是切片能否扛住多轮处理：TSA体系下一张切片要经历8–10轮"染色—成像—微波洗脱"，冰冻切片在这种流程中极易掉片、变形、坐标系漂移。 mIHC切片选型要点 ·
技术资料暂无技术资料 索取技术资料
