- 询价
- Cell Signaling Technology
- 7561
- USA
- 2025年07月15日
- 免疫荧光（IF）,流式细胞（Flow Cyt）
- 兔
- 人,大鼠,驴,斑马鱼,细菌,猪
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
COX IV (3E11) Rabbit mAb (Alexa Fluor 647 Conjugate)
- 抗原：
/
- 应用范围：
免疫荧光（IF）,流式细胞（Flow Cyt）
- 宿主：
兔
- 库存：
大量
- 级别：
详见MSDS文件
- 抗原来源：
/
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
人,大鼠,驴,斑马鱼,细菌,猪
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Glucose Metabolism
COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) #7561
PhosphoSitePlus ® protein, site, and accession data: COX4I1
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC F | H R Mk Z B Pg | Endogenous | Rabbit IgG |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human R=Rat Mk=Monkey Z=Zebrafish B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 7561:
- Flow , Immunofluorescence
Specificity / Sensitivity
COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total COX IV protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV protein.
Flow Cytometry
Flow cytometric analysis of HeLa cells using COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) (green) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with Alexa Fluor® 488 phalloidin (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated COX IV (3E11) Rabbit mAb #4850.
Background
Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.
- Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
- Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
- Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
- Barrientos, A. et al. (2002) Gene 286, 53-63.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验Antibody250µlIF-IC HCA4410SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4412SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F4413SAnti-rabbit IgG (H+L
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
valuable for analyzing human mitochondrial myopathies and related disorders.170,173,183,184 Alexa Fluor 488 and Alexa Fluor 594 conjugates of anti�COX subunit I are also available for direct staining of mitochondria (A21296, A21297; Figure 7.79). Mouse
Procedure for fixing and staining adherent cells for immunofluorescence
serum is for blocking purposes and should correspond to species that secondary antibody is developed in. For example, if you are using goat anti-rabbit 488 and goat anti-mouse 568, you should use 5 normal goat serum for blocking 8. Place 100uL
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