- 询价
- Cell Signaling Technology
- 7523
- USA
- 2026年05月29日
- F
- H,M,R,Mk,B,Pg,GP
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- 文献和实验
- 技术资料
- 抗体英文名：
IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (PE Conjugate)
- 抗原：
GST-IκBα fusion protein corresponding the amino-terminus of human IκBα protein
- 应用范围：
F
- 库存：
大量
- 供应商：
CST
- 适应物种：
H,M,R,Mk,B,Pg,GP
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 标记物：
PE
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine Pg=Pig GP=Guinea Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| F | H M R Mk B Pg GP | Endogenous | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (PE Conjugate) detects endogenous levels of total IκBα protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a GST-IκBα fusion protein corresponding the amino-terminus of human IκBα protein. Flow Cytometry
Flow cytometric analysis of HeLa cells, treated with Calyculin A #9902 and hTNF-α #8902 (blue) or untreated (green), using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (PE Conjugate). |
| Description | This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814. |
| Background | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
Antigen Design Sera Purification Tech Sheet
-terminal amino acid and vice versa for C-terminal sequences. Internal sequences can be coupled at either end. Another consideration for internal sequences is to acetlyate or amidate the unconjugated end as the sequence in the native protein molecule
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