- 询价
- Cell Signaling Technology
- 3476
- USA
- 2025年11月27日
- W
- H,M,R
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Tyr653/654 of human FGF receptor-1
- 应用范围：
W
- 适应物种：
H,M,R
- 供应商：
CST
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/300 ul (30 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (30 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H (M) (R) | Transfected Only | 120 to 145 | Mouse IgG2b |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb detects transfected levels of FGF receptors only when phosphorylated at tyrosines 653/654. The antibody cross-reacts slightly with activated PDGF and insulin/IGF-I receptors. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr653/654 of human FGF receptor-1. The corresponding sequence is identical in FGF receptor-2, -3 and -4. Western Blotting
Western blot analysis of extracts from COS cells overexpressing human FGF receptor-1, untreated or calf intestine phosphatase (CIP)-treated, using Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb. Overexpression of human FGF receptor-1 results in constitutive activation of the receptors (courtesy of Dr. Pamela Maher, Scripps Research Institute, California, personal communication). CIP treatment abolishes the reactivity of this antibody to FGF receptor-1. |
| Background | Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验pellets in 10 ml of 100 mM Tris buffer or Binding buffer. And pool into 50 ml Falcon tubes (try not to make subbles).Use 3.2 ml/cm 12-14,000 MW dialysis tubing. Heat tubing in 500 ml H2O in microwave. Not boiling. Rinse tubing in fresh H2O several times
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
and 10X PCR buffer. PCR program: 95¡C X 3'; 30 X (95¡C X 1', 55¡C X 1', 72¡C X 3');72¡C X 3' PCR for FGF9 KO mice Oligos... 5’ oligo: (JC46): 5’-GCA AGG GAG GGG AGT TGG ATA TAC C-3’ 3’ oligo: (JC42): 5’-CAG CCC AAG CTT TCG CGA GCT CG
技术资料暂无技术资料 索取技术资料
