- 询价
- Cell Signaling Technology
- 4893
- USA
- 2025年11月19日
- W, IHC-P, IF-IC, F
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Nanog (1E6C4) Mouse mAb
- 抗原：
recombinant human nanog protein
- 应用范围：
W, IHC-P, IF-IC, F
- 适应物种：
H
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 供应商：
CST
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H | Endogenous | 42 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Nanog (1E6C4) Mouse mAb detects endogenous levels of total nanog protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with recombinant human nanog protein. Western Blotting
Western blot analysis of extracts from NTERA and NCCIT cells using Nanog (1E6C4) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (1E6C4) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (1E6C4) Mouse mAb with biotin-based detection (left) or SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125 (right). Flow Cytometry
Flow cytometric analysis of HeLa cells (red) and NTERA2 cells (blue) using Nanog (1E6C4) Mouse mAb. IF-IC
Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog (1E6C4) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). |
| Background | Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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