- 询价
- Cell Signaling Technology
- 2329
- USA
- 2025年11月17日
- W
- Rabbit
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-PAR-4 (Thr163) Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Thr163 of human PAR-4 (Thr155 in rat and Thr156 in mouse)
- 应用范围：
W
- 宿主：
Rabbit
- 适应物种：
H,M,R,Mk
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (R) (Mk) | Endogenous | 43 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-PAR-4 (Thr163) Antibody detects endogenous levels of PAR-4 when phosphorylated at Thr163 (Thr163 corresponds to human sequence and is equivalent to Thr155 in rat and Thr156 in mouse). |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr163 of human PAR-4 (Thr155 in rat and Thr156 in mouse). Antibodies were purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or forskolin-treated (30 μM), using Phospho-PAR-4 (Thr163) Antibody (upper) or total PAR-4 Antibody #2328 (lower). Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A (100 nM, 5 minutes), using Phospho-PAR-4 (Thr163) Antibody (upper) or total PAR-4 Antibody #2328 (lower). |
| Background | PAR-4 (prostate apoptosis response-4) was identified as a protein that is upregulated in prostate tumor cells undergoing apoptosis (1). Additionally, in parallel studies PAR-4 was found in the yeast two-hybrid system to bind to the Wilms' tumor suppressor protein WT1 and may modulate WT1-medated transcriptional activation (2). PAR-4 contains a leucine zipper domain and a death domain and has been implicated as an effector of apoptosis during tumorigenesis as well as in neurodegenerative disorders (3,4). PAR-4 is widely expressed in normal tissues but can be downregulated in some tumor types. The mechanism of PAR-4 mediated apoptosis regulation appears to be complex and dependent on the cellular context. Studies have indicated roles for PAR-4 in activation of the Fas-FADD-caspase-8 pathway as well as inhibition of the NF-κB pro-survival pathway (5-7). Its activity is likely to depend on the cellular context and post-translational modifications. For instance, phosphorylation of PAR-4 by Akt prevents its nuclear translocation thereby promoting cell surivival (8). In contrast, phoshorylation of rat PAR-4 at T155 by PKA appears to positively regulate its apoptotic activity (9).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验in goat serum 1 x 60' primary antibody (rabbit-anti-PAR-1 in 1% BSA, 10% serum in PBS) -- optionally incubated 4 hours @ 16 degrees C 1 x 10' wash in PBS 1 x 10' wash in PBS + 0.5% Tween 1 x 10' wash in PBS
人可溶性CD163分子(sCD163)ELISA试剂盒 说明书
人可溶性CD163 分子 ( sCD163 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 sCD163 单抗包被于酶标板上，标准品和样品中的 sCD163与单抗结合，加入生物素化的抗人 sCD163 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
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