Phospho-NDRG1 (Ser330) Antibod

3506:

Immunoprecipitation , Western Blotting

Phospho-NDRG1 (Ser330) Antibody detects endogenous levels of NDRG1 when phosphorylated at Ser330.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser330 of NDRG1. Antibodies are purified by protein A and peptide affinity chromaography.

Western Blotting

Western blot analysis of extracts from Jurkat cells, treated with Calyculin A #9902 alone (100 nM for 20 min) or together with λ-phosphatase, using Phospho-NDRG1 (Ser330) Antibody.

N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3.

Phospho-NDRG1 (Ser330) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan^® , CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser330 was discovered using an Akt substrate antibody. Please visit PhosphoSitePlus^® , CST's modification site knowledgebase, at www.phosphosite.org for more information.

1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
2. Zhou, D. et al. (1998) Cancer Res 58, 2182-9.
3. van Belzen, N. et al. (1997) Lab Invest 77, 85-92.
4. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44.
5. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8.
6. Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105.
7. Stein, S. et al. (2004) J Biol Chem 279, 48930-40.
8. Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42.
9. Nishio, S. et al. (2008) Cancer Lett 264, 36-43.
10. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58.
11. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56.
12. Taketomi, Y. et al. (2007) J Immunol 178, 7042-53.
13. Murray, J.T. et al. (2004) Biochem J 384, 477-88.

• Hoang, B. et al. (2010) Blood 116, 4560-8. Applications: Western Blotting

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For Research Use Only. Not For Use In Diagnostic Procedures.