Applications Key: W=Western Blotting IP=Immunoprecipitation Reactivity Key: H=Human Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
DDX4 (2F9H5) Mouse mAb detects endogenous levels of total DDX4 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a purified recombinant fragment of human DDX4.
Western Blotting
Western blot analysis of extract from human testis tissue using DDX4 (2F9H5) Mouse mAb.
Background
DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells (1). This enzyme is specific to germ cells and is necessary for germ cell development (2). Mouse DDX4 was shown to interact with Dicer suggesting its role in microRNA-mediated RNA silencing (1).