- 询价
- Cell Signaling Technology
- 5447
- USA
- 2025年07月16日
- 免疫荧光（IF）,流式细胞（Flow Cyt）
- 小鼠
- 人
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
EpCAM (VU1D9) Mouse mAb (Alexa Fluor 647 Conjugate)
- 抗原：
/
- 应用范围：
免疫荧光（IF）,流式细胞（Flow Cyt）
- 宿主：
小鼠
- 供应商：
CST
- 库存：
大量
- 级别：
详见MSDS文件
- 适应物种：
人
- 抗原来源：
/
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Cytoskeletal Signaling
EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 647 Conjugate) #5447
PhosphoSitePlus ® protein, site, and accession data: EPCAM
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC F | H | Endogenous | Mouse IgG1 |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5447:
- Flow , Immunofluorescence
Specificity / Sensitivity
EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total EpCAM protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with NCI-H69 small cell lung carcinoma cells.
Flow Cytometry
Flow cytometric analysis of unpermeabilized HT-29 cells using EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 647 Conjugate) (green) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 647 Conjugate) #4843 (red).
IF-IC
Confocal immunofluorescent analysis of HT-29 cells using EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb #9725 (green).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated EpCAM (VU1D9) Mouse mAb #2929.
Background
Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+ -independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). One of the first tumor-associated antigens discovered, EpCAM has long been a marker of epithelial and tumor tissue. Because of its high expression in cancer, EpCAM has become a therapeutic target for human solid tumors of epithelial lineage (reviewed in 2,3).
- Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
- Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
- Armstrong, A. and Eck, S.L. Cancer Biol Ther 2, 320-6.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 5198 EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 2929 EpCAM (VU1D9) Mouse mAb
- 4843 Mouse (MOPC-21) mAb IgG1 Isotype control (Alexa Fluor® 647 Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验Antibody250µlIF-IC HCA4410SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4412SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F4413SAnti-rabbit IgG (H+L
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
4.5 (without removing prehybridization solution.) C. Repeat the addition of the 2XSSC wash twice more. Remove the mix and wash twice, 30 min each time, in 2XSSC pH7/0.1% CHAPS 70 °C. Wash twice, 10 min each, in Maleic Acid Buffer ( MAB; 100 mM maleic acid
WHOLE MOUNT IN SITU HYBRIDIZATION.Mouse Embryos
expressed in the anterior primitive endoderm of the mouse gastrula" Mechanism of Development, in press - 1997. Modified from the protocol of Harland (1991) and Current Protocols in Molecular Biology. Preparation of the probe.
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