β-Catenin (L87A12) Mouse mAb产品图

β-Catenin (L87A12) Mouse mAb

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  • Cell Signaling Technology已认证
  • 2698
  • USA
  • 2026年05月28日
  • W, IP
  • H,M,R,Mk
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体英文名:

      β-Catenin (L87A12) Mouse mAb

    • 抗原:

      synthetic peptide corresponding to residues surrounding Asp56 of human β-catenin

    • 应用范围:

      W, IP

    • 保质期:

      详见说明书

    • 供应商:

      CST

    • 库存:

      大量

    • 适应物种:

      H,M,R,Mk

    • 级别:

      详见MSDS文件

    • 是否单克隆:

      1

    • 保存条件:

      -20°c

    • 规格:

      100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting  IP=Immunoprecipitation
    Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Isotype
    W IP H M R Mk Endogenous 92 Mouse IgG1
    Protocols
    Specificity / Sensitivity

    β-Catenin (L87A12) Mouse mAb detects endogenous levels of total β-catenin protein.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp56 of human β-catenin.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from various cell lines using β-Catenin (L87A12) Mouse mAb.

    Background

    β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

    1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
    2. Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
    3. Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
    4. Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
    5. Lin, C. et al. (2002) Cell 108, 837-847.
    6. Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
    7. Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
    8. Morin, P.J. (1997) Science 275, 1787-1790.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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    图标文献和实验
    相关实验
    • 别再只测β‑catenin!完整搞懂Wnt经典与非经典信号通路

      领域。   02 Wnt信号通路分子组成   Wnt配体位于信号系统的上游。哺乳动物中目前已鉴定出19种Wnt蛋白,它们属于分泌型信号蛋白,可以由产生Wnt的细胞释放,并作用于邻近或特定距离的靶细胞。Wnt释放后,需要通过细胞膜上的受体识别并传递信号。其中最重要的一类受体是Frizzled(FZD)家族。哺乳动物中共有10种FZD受体,它们都是具有七次跨膜结构的受体蛋白。在经典Wnt/β-catenin信号中

    • Purification of mAb (IgG)

      7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml

    • Simultaneous Immunofluorescence Detection of Cadherin and -Catenin in Mouse Tissue Sections

      Both Cell-Cell adhesion and secreted signaling molecules are involved in the regulation of normal tissue development and maintenance. β-catenin seems to link cadherin-mediated Cell-Cell adhesion and Wnt signaling. Thus, its activity

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