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Phospho-c-Jun (Ser63) (54B3) Rabbit mAb
synthetic phosphopeptide corresponding to residues around Ser63 of human c-Jun
W, IHC-P
H,M,R
详见MSDS文件
详见说明书
CST
大量
1
-20°c
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
规格: | 产品价格: | ¥请询价 | |
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规格: | 40 ul (4 western blots) | 产品价格: | ¥请询价 |
规格: | 100 ul (10 western blots) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
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W IHC-P | H M R | Endogenous | 48 | Rabbit IgG |
Protocols |
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Specificity / Sensitivity | Phospho-c-Jun (Ser63) (54B3) Rabbit mAb detects endogenous levels of c-Jun only when phosphorylated at serine 63. |
Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser63 of human c-Jun. Western BlottingWestern blot analysis of extracts from untreated or anisomycin-treated C6 cells, or untreated or UV-treated 293 cells, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb (upper) or c-Jun (60A8) Rabbit mAb #9165 (lower). IHC-P (paraffin)Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb in the presence of control peptide (left) or Phospho-c-Jun (Ser63) II Blocking Peptide (#1020) (right). IHC-P (paraffin)Immunohistochemical analysis of paraffin-embedded human breast carcinoma untreated (left) or lambda phosphatase-treated (right), using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb. IHC-P (paraffin)Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear localization, using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb. IHC-P (paraffin)Immunohistochemical analysis of paraffin-embedded NIH/3T3 cell pellets, control (left) or anisomycin-treated (right), using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb. IHC-P (paraffin)Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells untreated (left) or anisomycin-treated (right), using Phospho-c-Jun (Ser63) (54B3) Rabbit mAb. |
Background | c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).
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Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know! |
Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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