- 询价
- Cell Signaling Technology
- 4891
- USA
- 2025年11月19日
- W, IF-IC
- Rabbit
- H,M,R
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- 详细信息
- 技术资料
- 抗体英文名：
UBE1a/b Antibody
- 抗原：
synthetic peptide surrounding Thr800 of human UBE1
- 应用范围：
W, IF-IC
- 宿主：
Rabbit
- 库存：
大量
- 供应商：
CST
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 适应物种：
H,M,R
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R | Endogenous | 110, 117 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | UBE1a/b Antibody detects endogenous levels of total UBE1a and UBE1b proteins. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Thr800 of human UBE1. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from K562 and SK-N-MC cells using UBE1a/b Antibody. IF-IC
Confocal immunofluorescent analysis of SK-N-MC cells using UBE1a/b Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
| Background | Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa (5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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