Phospho-Lamin A/C (Ser22) Anti

2026:

Immunofluorescence , Western Blotting

Phospho-Lamin A/C (Ser22) Antibody detects endogenous levels of lamin A/C only when phosphorylated at Ser22.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser22 of human lamin A/C protein.

Western Blotting

Western blot analysis of extracts from HeLa and NIH/3T3 cells, hydroxyurea-treated (4 mM, 20 hours) to induce G1/S phase or paclitaxel-treated (100 nM, 20 hours) or nocodazole-treated (100 ng/ml, 20 hours) to induce G2/M phase, using Phospho-Lamin A/C (Ser22) Antibody (upper) or Lamin A/C Antibody #2032 (lower) as a loading control.

Western Blotting

Western blot analysis of extract from G2/M phase HeLa cells untreated or treated with phosphatase, using Phospho-Lamin A/C (Ser22) Antibody (upper) or Lamin A/C Antibody (lower) as loading control.

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Phospho-Lamin A/C (Ser22) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

Phosphorylation of Lamin A/C at Ser22 was identified in vivo in several cell lines by mass spectrometry analysis in proteomic screens. The surrounding sequence is a typical MAPK/CDK phosphorylation motif, which implicates a role in the cell cycle and mitosis (7-11).

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
7. Lowery, D.M. et al. (2007) EMBO J 26, 2262-73.
8. Molina, H. et al. (2007) Proc Natl Acad Sci USA 104, 2199-204.
9. Beausoleil, S.A. et al. (2006) Nat Biotechnol 24, 1285-92.
10. Nousiainen, M. et al. (2006) Proc Natl Acad Sci USA 103, 5391-6.
11. Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci USA 101, 12130-5.

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For Research Use Only. Not For Use In Diagnostic Procedures.