Bif-1 Antibody

4467:

Immunoprecipitation , Western Blotting

Bif-1 Antibody recognizes endogenous levels of total Bif-1 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly129 of human Bif-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell lines using Bif-1 Antibody.

Bif-1/SH3GLB1/Endophilin-B1 is a member of the endophilin B family that was originally identified as a Bax binding protein through yeast two-hybrid screening (1,2). Bif-1 does not have significant homology to other Bcl-2 family members, but rather contains an N-terminal Bin-Amphiphysin-Rvs (BAR) domain, typically involved in membrane dynamics, and a C-terminal SH3 domain. Overexpression of Bif-1 promotes Bax conformational change and apoptosis (2,3). Likewise, loss of Bif-1 inhibits Bax and Bak activation, cytochrome c release, and caspase activation (3). Bif-1 is localized to membranes of intracellular organelles and has been suggested to play a role in membrane dynamics, including that during autophagy. Bif-1 directly binds to UVRAG, forming a complex with Beclin-1, resulting in increased PI3-kinase class III/Vps34 activity required for autophagosome maturation (4). Inhibition of GSK-3β, as seen during nutrient deprivation, results in increased expression of Bif-1, and can contribute to autophagic cell death (5). Research studies have shown that loss of Bif-1 promotes tumorigenesis, and decreased expression of Bif-1 has been noted in several cancer types (6-11).

1. Pierrat, B. et al. (2001) Genomics 71, 222-34.
2. Cuddeback, S.M. et al. (2001) J Biol Chem 276, 20559-65.
3. Takahashi, Y. et al. (2005) Mol Cell Biol 25, 9369-82.
4. Takahashi, Y. et al. (2007) Nat Cell Biol 9, 1142-51.
5. Yang, J. et al. (2010) J Cell Sci 123, 861-70.
6. Coppola, D. et al. (2011) Pancreas 40, 433-7.
7. Coppola, D. et al. (2008) Cancer 113, 2665-70.
8. Coppola, D. et al. (2008) Clin Genitourin Cancer 6, 117-21.
9. Kim, S.Y. et al. (2008) Pathology 40, 553-7.
10. Takahashi, Y. et al. (2007) Nat Cell Biol 9, 1142-51.
11. Lee, J.W. et al. (2006) Pathology 38, 312-5.

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• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.