- ￥2800 - 6800
- Sino Biological
- 39.06 pg/ml
- 北京
- SEK51003
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 检测范围：
39.06 pg/ml
- 检测方法：
ELISA
- 应用：
ELISA
- 适应物种：
Mouse
- 标记物：
HRP
- 样本：
见说明书
- 规格：
5 Plates/15 Plates
| 规格： | 5 Plates | 产品价格： | ￥2800.0 |
|---|---|---|---|
| 规格： | 15 Plates | 产品价格： | ￥6800.0 |
反应种属：Mouse
试剂对靶点：ERBB3
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：39.06-2500
试剂对标记类型：HRP
包被抗体：1 mg/mL of rabbit anti-Mouse ERBB3/HER3 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 51003-R128 )
检测抗体：0.2 mg/mL of rabbit anti-Mouse ERBB3/HER3 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 51003-R138)
试剂灵敏度：The minimum detectable dose of Mouse ERBB3/HER3 was determined to be approximately 39.06 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Mouse ERBB3/HER3 ELISA Pair Set is for the quantitative determination of Mouse ERBB3/HER3. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Mouse ERBB3/HER3 coated on a 96-well plate. Standards and samples are added to the wells, and any Mouse ERBB3/HER3 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Mouse ERBB3/HER3 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Mouse ERBB3/HER3 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：39.06 pg/ml
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
, pour out 2 x petri dishes of PBS in the hood. Pregnant mouse is killed by cervical dislocation. (This is not done in the hood but on clean benchcote). Lay mouse out on its back and swab belly with 70% ethanol. With a pair of scissors (not sterile) nip
Euthanasia Euthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distress
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