Mouse EpCAM / TROP-1 / TACSTD1 ELISA Pair Set(产品说明)
反应种属:Mouse
试剂对靶点:EpCAM
试剂对应用:ELISA
试剂对保存条件:Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围:78.13-5000
试剂对标记类型:HRP
包被抗体:1.0 mg/mL of rabbit anti-mouse EPCAM monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 50591-R052)
检测抗体:0.5 mg/mL rabbit anti-mouse EPCAM monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 50591-R002)
试剂灵敏度:The minimum detectable dose of Mouse EpCAM / TROP-1 / TACSTD1 was determined to be approximately 78 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理:The Mouse EpCAM / TROP-1 / TACSTD1 ELISA Pair Set is for the quantitative determination of Mouse EpCAM / TROP-1 / TACSTD1.This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for EpCAM / TROP-1 / TACSTD1 coated on a 96-well plate. Standards and samples are added to the wells, and any EpCAM / TROP-1 / TACSTD1 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-EpCAM / TROP-1 / TACSTD1 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of EpCAM / TROP-1 / TACSTD1 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限:78 pg/ml