- ￥2800 - 6800
- Sino Biological
- 156 pg/ml
- 北京
- SEK50071
- 2025年08月12日
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- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 检测范围：
156 pg/ml
- 检测方法：
ELISA
- 应用：
ELISA
- 适应物种：
Mouse
- 标记物：
HRP
- 样本：
见说明书
- 规格：
5 Plates/15 Plates
| 规格： | 5 Plates | 产品价格： | ￥2800.0 |
|---|---|---|---|
| 规格： | 15 Plates | 产品价格： | ￥6800.0 |
反应种属：Mouse
试剂对靶点：FGFR3
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：156-10000
试剂对标记类型：HRP
包被抗体：1 mg/mL of rabbit anti-Mouse FGFR3 / CD333 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50071-R042)
检测抗体：0.2 mg/mL of rabbit anti-Mouse FGFR3 / CD333 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 50071-R046)
试剂灵敏度：The minimum detectable dose of Mouse FGFR3 / CD333 was determined to be approximately 156 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Mouse FGFR3 / CD333 ELISA Pair Set is for the quantitative determination of Mouse FGFR3 / CD333. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Mouse FGFR3 / CD333 coated on a 96-well plate. Standards and samples are added to the wells, and any Mouse FGFR3 / CD333 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Mouse FGFR3 / CD333 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Mouse FGFR3 / CD333 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：156 pg/ml
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文献和实验ELISPOT (Enzyme-linked ImmunoSPOT)
PBMCs activated with PMA/Ionomycin for 24 hrs. Reference Tables Table 1: Mouse ELISPOT Ready-Set-Go! Sets & Antibody Selection Quick Guide Mouse ELISPOT Ready-Set-Go! Sets & Antibody Selection Quick Guide
免疫组化技术-Immunohistochemical Protocols
in 2-methylbutane (Aldrich, Milwaukee, WIS #M3,263-1) or on powdered dry ice. There are advantages and disadvantages to both tissue preparations: perfusion needs a special set-up, special expertise and is more time consuming. However, most antigens
【共享】流式 Overview of Flow Cytometry
is dependent upon the ability to distinguish “signal” (positive fluorescence) from noise (background) for any set of probes and target cells being studied. While a signal equivalent to 5000 molecules of fluorescein is easily resolvable on fresh mouse thymocytes
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