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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
3个月
- 英文名:
Rat Cyp3a9 Gene ORF cDNA clone in cloning vector
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1 Unit
| 货号: | 表达载体: |
| RG82890-U | pUC19 Vector |
| RG82890-ACGLN | pLV-C-GFPSpark |
Rat Cyp3a9 Gene ORF cDNA clone in cloning vector产品信息
基因靶点:Cyp3a9
反应种属:Rat
应用说明:
cDNA描述:Full length Clone DNA of Rat cytochrome P450, family 3, subfamily a, polypeptide 9
| 货号: | RG82890-U |
| 产品名称: | Rat Cyp3a9 Gene ORF cDNA clone in cloning vector |
| NCBI 参考序列号: | NM_147206.2 |
| 序列长度: | 1512 |
| 表达载体: | pUC19 Vector |
| cDNA描述: | Full length Clone DNA of Rat cytochrome P450, family 3, subfamily a, polypeptide 9 |
| 货号: | RG82890-ACGLN |
| 产品名称: | Rat Cyp3a9 Gene Lentiviral ORF cDNA expression plasmid, C-GFPSpark tag |
| NCBI 参考序列号: | NM_147206.2 |
| 序列长度: | 1512 |
| 表达载体: | pLV-C-GFPSpark |
| cDNA描述: | Full length Clone DNA of Rattus norvegicus cytochrome P450, family 3, subfamily a, polypeptide 9 |
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文献和实验In Vitro Assays for Induction of Drug Metabolism
and obviates the need to harvest cells and prepare subcellular fractions prior to the assay of enzyme activities. The induction of CYP1A and CYP3A forms in human and rat hepatocytes can be determined by measurement of 7-ethoxyresorufin O -deethylase
Using the DFCI Gene Index Databases for Biological Discovery
by Radiation Hybrid Map Location (for Human, Mouse, and Rat Only) Alternate Protocol 4: Search Gene Expression by Library Annotation Alternate Protocol 5: Searching by Metabolic Pathway Basic
我的题目是鸡Mx基因的克隆,我的策略是先根据该基因家族(dynamin family)的氨基酸保守区设计简并引物克隆出该基因cDNA的部分片段,然后根据该片段序列设计基因特异性引物,利用RACE技术得到cDNA的3‘和5’末端,然后根据末端序列设计引物扩增出该基因全长cDNA. 我用简并引物从鸡cDNA中扩增出一个片段,约250bp, 测序后拿来BLAST时却发现,与之同源性最高的并不是其它的Mx基因,而是人的某一个基因,比对的结果中与该序列同源性较高的有人及鼠Mx基因,但是同源性并不
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