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- 详细信息
- 文献和实验
- 技术资料
- 提供商:
简石生物技术(浙江)有限公司
- 服务名称:
基因定点突变( Site-directed mutagenesis)
服务内容:根据客户的突变要求,突变模板质粒中单个或多个碱基,保留客户原始序列的完整性。
样品要求:1ug质粒型表达载体或其它载体(如pENTR载体)中已构建入目的基因,该目的基因需经测序证明序列正确(请提供测序彩图)。请提供目的基因Genebank 序列号以供比对;请提供载体的性质、抗性及构建方向,测序引物名称、序列。
终产品:纯化并经测序验证的质粒,提供测序彩图。
服务周期:单个位点1-2周。
价格
| 定点突变DNA序列(模板2kb内) | 突变1个位点 | ¥1000/位点 | 3-4周⑤ | |
| 2≤突变位点≤5 | ¥800/位点 | |||
| 突变位点>5 | ¥800/位点 | |||
| 定点突变DNA序列(模板大于2kb) | 突变1个位点 | ¥1500/位点 | 3-4周⑤ | |
| 2≤突变位点≤5 | ¥1200/位点 | |||
| 突变位点>5 | ¥1000/位点 | |||
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文献和实验The ability to create defined mutations and to generate precise fusions of protein domams by modifications at the DNA level is now an accepted and routine technique in molecular biology This ability has developed over the past 10–l5 years
Multiple Site-Directed Mutagenesis
Site-directed mutagenesis techniques allow selective engineering of gene sequences. In in vitro site-directed mutagenesis, base alterations are introduced into a target sequence by incorporating DNA base changes within a primer utilized
Proteoglycan: Site Mapping and Site-Directed Mutagenesis
flanked by a C-terminal glycine and proximal N-terminal acidic amino acids; however, not all simple Ser-Gly motifs constitute a modification site. Here we present a rapid method for cloning small, defined segments of putative proteoglycan attachment sites
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