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- 文献和实验
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- 保存条件:
-20℃ to -80℃
- 保质期:
3个月
- 英文名:
Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, C-GFPSpark tag
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1 Unit
| 货号: | 表达载体: |
| MG52927-ACG | pCMV3-C-GFPSpark |
| MG52927-ANG | pCMV3-N-GFPSpark |
| MG52927-CF | pCMV3-C-FLAG |
| MG52927-CH | pCMV3-C-His |
| MG52927-CM | pCMV3-C-Myc |
| MG52927-CY | pCMV3-C-HA |
| MG52927-G | PGEM-T Vector |
| MG52927-NF | pCMV3-N-FLAG |
| MG52927-NH | pCMV3-N-His |
| MG52927-NM | pCMV3-N-Myc |
| MG52927-NY | pCMV3-N-HA |
| MG52927-UT | pCMV3-untagged |
| MG52927-ACR | pCMV3-C-OFPSpark |
| MG52927-ANR | pCMV3-N-OFPSpark |
| MG52927-ACGLN | pLV-C-GFPSpark |
Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, C-GFPSpark tag产品信息
基因靶点:SerpinC1
反应种属:Mouse
应用说明:Stable or Transient mammalian expression
cDNA描述:Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 with C terminal GFPSpark tag.
| 货号: | MG52927-ACG |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, C-GFPSpark tag |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1398 |
| 表达载体: | pCMV3-C-GFPSpark |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 with C terminal GFPSpark tag. |
| 货号: | MG52927-ANG |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, N-GFPSpark tag |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1398 |
| 表达载体: | pCMV3-N-GFPSpark |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 with N terminal GFPSpark tag. |
| 货号: | MG52927-CF |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, C-Flag tag |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1437 |
| 表达载体: | pCMV3-C-FLAG |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 with C terminal Flag tag. |
| 货号: | MG52927-CH |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, C-His tag |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1443 |
| 表达载体: | pCMV3-C-His |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 with C terminal His tag. |
| 货号: | MG52927-CM |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, C-Myc tag |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1398 |
| 表达载体: | pCMV3-C-Myc |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 with C terminal Myc tag. |
| 货号: | MG52927-CY |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, C-HA tag |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1398 |
| 表达载体: | pCMV3-C-HA |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 with C terminal HA tag. |
| 货号: | MG52927-G |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone in cloning vector |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1398 |
| 表达载体: | PGEM-T Vector |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1. |
| 货号: | MG52927-NF |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, N-Flag tag |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1398 |
| 表达载体: | pCMV3-N-FLAG |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 with N terminal Flag tag. |
| 货号: | MG52927-NH |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, N-His tag |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1398 |
| 表达载体: | pCMV3-N-His |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 with N terminal His tag. |
| 货号: | MG52927-NM |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, N-Myc tag |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1398 |
| 表达载体: | pCMV3-N-Myc |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 with N terminal Myc tag. |
| 货号: | MG52927-NY |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, N-HA tag |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1398 |
| 表达载体: | pCMV3-N-HA |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 with N terminal HA tag. |
| 货号: | MG52927-UT |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1398 |
| 表达载体: | pCMV3-untagged |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1. |
| 货号: | MG52927-ACR |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, C-OFPSpark tag |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1398 |
| 表达载体: | pCMV3-C-OFPSpark |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 with C terminal OFPSpark / RFP tag. |
| 货号: | MG52927-ANR |
| 产品名称: | Mouse Antithrombin III/ATIII Gene ORF cDNA clone expression plasmid, N-OFPSpark tag |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1398 |
| 表达载体: | pCMV3-N-OFPSpark |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 with N terminal OFPSpark / RFP tag. |
| 货号: | MG52927-ACGLN |
| 产品名称: | Mouse Antithrombin III/ATIII Gene Lentiviral ORF cDNA expression plasmid, C-GFPSpark tag |
| NCBI 参考序列号: | NM_080844.4 |
| 序列长度: | 1398 |
| 表达载体: | pLV-C-GFPSpark |
| cDNA描述: | Full length Clone DNA of Mus musculus serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1. |
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文献和实验。 SuperScript cDNA文库使用pCMVSPORT构建,有几种人组织来源可供选择,这些文库有很大一部分是全长的插入片段,可以完全代表来源mRNA。 4、入门克隆的切入点——克隆资源 您可以从与10000个人类基因相关的35000个克隆中进行选择,这些克隆的70%以上是全长序列的。这些克隆来源于使用特殊的高级cDNA文库构建技术、oligo dT引物以及SuperScript II 反转录酶所构建的文库。许多克隆来源于I.M.A.G.E.协会,NCI CGAP 项目,ResGen库或UltimateORF
的温度。在18°C,选择优化的蛋白质表达条件,这可能会增加表达蛋白质的溶解度。故障排除尝试较低的生长温度和较低的诱导剂浓度可以避免包涵体的形成,并有助于一些不正确的二硫键形成和疏水蛋白的情况。为了保护蛋白质,我们可以在培养后向培养基中加入蛋白酶抑制剂,并在冰上破碎细胞以避免降解。在大肠杆菌裂解物中在天然条件下的蛋白质纯化Trx•Tag™是移植到重组蛋白上的肽序列,可以通过肠激酶去除。这种蛋白质标签是一种增稠标签,可以帮助蛋白质正确折叠并防止它们沉淀。由重组pET-32α(+)载体表达的融合蛋白也含有
培养箱中培养。 2、实验结果 图:1×10^6/孔细胞,5μg anti-mouse CD3与CD28刺激3天后细胞形成明显克隆团 CD3/CD28 Streptamer® 多聚体法 1、实验原理 使用低亲和力的抗 CD3 和抗CD28的抗体 Fab 片段(非传统全长抗体),结合上 Twin-Strep-tag 亲和标签,形成 CD3 Fab-Strep 和 CD28 Fab-Strep。再结合可溶性蛋白多聚体 Strep-Tactin®,在亲和力作用下,即可与 TCR 和 CD28 共刺激
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