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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
3个月
- 英文名:
Human IL12RB1 Gene Lentiviral ORF cDNA expression plasmid, C-GFPSpark tag
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1 Unit
基因靶点:IL12RB1
反应种属:Human
应用说明:
cDNA描述:Full length Clone DNA of Homo sapiens interleukin 12 receptor, beta 1.
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文献和实验In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle Tissue
to background level within 4 weeks. Katushka expression was not as stable as GFP expression, which was detectable for 8 weeks. Depth and 3D analysis proved that the expression was located in the target muscle. In vivo bio-imaging using the novel Katushka
. RNA 表达方法:siRNA 表达系统或者 shRNA 表达系统当使用原代培养细胞或者非分裂细胞等难以转染的细胞时,除了慢病毒载体外,也可以使用比病毒更安全的 GenomONETM -Neo EX HVJ-E 仙台病毒包膜转染试剂,无需包装病毒,直接将 siRNA 与病毒包膜溶液混合,按照常规流程转染,即可达到病毒转染的效果。缩短实验周期,根据结果,灵活更换 siRNA 序列。 3.2 siRNA 序列设计 了解了 RNAi 的干扰机制后,需要设计既能避免脱靶又可以高效敲除
Imaging of Endogenous RNA Using Genetically Encoded Probes
and may allow for elucidating novel functions of localized RNAs and understanding their dynamics in living cells. Curr. Protoc. Chem. Biol. 3:27?37 © 2011 by John Wiley & Sons, Inc. Keywords: RNA; imaging; GFP; fluorescence; molecular beacon
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