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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
3个月
- 英文名:
Human B3GNT1 Gene ORF cDNA clone expression plasmid, N-His tag
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1 Unit
基因靶点:B4GAT1
反应种属:Human
应用说明:Stable or Transient mammalian expression
cDNA描述:Full length Clone DNA of Human beta-1,4-glucuronyltransferase 1 with N terminal His tag.
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文献和实验Using the DFCI Gene Index Databases for Biological Discovery
. Genet. 10:369‐371. Cariaso, M., Folta, P., Wagner, M., Kuczmarski, T., and Lennon, G. 1999. IMAGEne I: Clustering and ranking of I.M.A.G.E. cDNA clones corresponding to known
一,酵母表达系统毕赤酵母表达系统(invitrogen公司):分泌型质粒pPIC9K,宿主菌毕赤酵母GS115分泌型质粒pPICZalpha (甲醇诱导)宿主菌毕赤酵母x33分泌型质粒pHIL-S1二,原核表达质粒表达宿主菌为BL21(DE3)系列带His标签(N端):PET5-(a)氨苄抗性带His标签(N端):PET28-(a)卡那抗性带His标签(C端):PET24-(b)卡那抗性带His标签(C端):PET21-(a)氨苄抗性带His标签(N端)+TrxA(硫氧还蛋白):PET-32
PCR-Based Full-Length cDNA Cloning Utilizing the Universal-Adaptor/Specific DOS Primer-Pair Strategy
Biological systems are often influenced by molecules that are neither present in vast quantities or easily purified to homogeneity from other cellular constituents. The development of simple, efficient molecular cloning systems coupled
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