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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
3个月
- 英文名:
Human CCN3 / NOV (IGFBP9) Gene ORF cDNA clone expression plasmid, N-HA tag
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1 Unit
基因靶点:NOV
反应种属:Human
应用说明:Stable or Transient mammalian expression
cDNA描述:Full length Clone DNA of Human nephroblastoma overexpressed gene (NOV) with N terminal HA tag.
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文献和实验cDNA Expression Cloning in Mammalian Cells
protocols for expression cloning in mammalian cells. Either calcium phosphate? or liposome?mediated transfection of mammalian cells, or virus infection and liposome?mediated transfection are used to screen pools derived from a cDNA library. cDNA pools
24. Check the gene expression of each clone by standard reverse transcriptase polymerase chain reaction (RT-PCR). See Amplification of cDNA Generated by Reverse Transcription of mRNA (Sambrook and Russell 2006b ).
Homology Cloning of cDNA or Genomic DNA
. This unit describes conditions that have been used to clone and identify novel genes and cDNA clones using low?stringency hybridization of known probes to membranes that contain libraries of bacterial or bacteriophage DNA.
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