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- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human LSD1 Gene ORF cDNA clone expression plasmid, N-HA tag
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1 Unit
基因靶点:KDM1A
反应种属:Human
应用说明:Stable or Transient mammalian expression
cDNA描述:Full length Clone DNA of Human lysine (K)-specific demethylase 1A with N terminal HA tag.
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文献和实验Co-Expression of human DNA primase p49-p58 subunits
.Note: You don't have to shake (the Fanning lab simply transfers their flasks to a RT H2O bath),but I found that it does improve expression levels. 6.Harvest by centrifugation,5000 rpm,10 min.,4℃.Freeze pellets at -80℃. Purification of p49-p58 human
Introduction of human 14-3-3 proteins
and their secondary structure was shown by circular dichroism to be identical to wild-type protein,and expression of N-terminally-deleted epsilon 14-3-3 protein showed that the N-terminal 26 amino acids are important for dimerization.Intact 14-3-3 is a potent
Purification of Human Multiprotein Complexes using OneSTrEP Technology
Introduction Here we describe a strategy for isolation of multiprotein complexes from human HeLa S3 cells in a scale and purity optimized for characterization by mass spectrometry. For this purpose, we use stably expressed
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