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- 详细信息
- 文献和实验
- 技术资料
- 细胞类型:
干细胞
- 供应商:
上海逍鹏生物科技有限公司
- 规格:
10 mg
Product Description
Highly potent inhibitor of p160-Rho-associated coiled protein kinase (ROCK),
Y27632 dihydrochloride is a highly specific inhibitor of Rho-associated coiled-coil protein kinase (ROCK) activity as a cell-permeable, ATP-competitive inhibitor of both ROCK1 (Ki of 220 nM) and ROCK2 (Ki 300 nM) (Ishizaki, et al.). Human embryonic stem cell (ESC) survival is improved through enhanced attachment following single cell dissociation and subsequently after FACS analysis or cryopreservation in both feeder-dependent and -independent cultures (Emre, et al.). The enhanced cell attachment and thus recovery is boosted through decreased dissociation-induced apoptosis (Watanabe, et al.).
Product Synonyms:
Y-27632 dihydrochloride, Y-27632, Rock Inhibitor
Constructed from renowned protocols, Biological Industries' selection of small molecules will streamline workflows for research in diabetes, neural disorders, and cardiovascular disease.
产品官网链接:https://www.bioind.com/worldwide/y27632/
想了解更多种类的小分子,请点击官网:
https://www.bioind.com/worldwide/products/stem-cell-research/small-molecules/
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文献和实验Preparation of Carboxypeptidase Y and Its Properties
while high efficiency habit in the scientific research. 【Requirement】 1. Use bread yeast as the original material to extract and condense carboxypeptidase Y, assay its biochemical properties. 2. Through gathering relevant published papers
用喹吖因(quinacrine)或喹吖因芥子( quin- acrine mustard)染色,然后用荧光显微镜观察,在人的 Y染色体的长臂末端处能看到很强的荧光。在分裂期间的细胞核中也很容易看出这种荧光,因此把它叫做 Y染色质或 Y小体。由于 Y小体只有在男性中才能观察到,所以可用于人的性别鉴定。最近也已被奥林匹克运动会用于检查运动员的性别。然而这种现象只在人和大猩猩中观察到,在黑猩猩和猩猩等其他动物中, Y小体并不发出特别强的荧光。
每年到修改论文的时候,发现很多同学不懂图形和数据处理,出来的图形惨不忍睹。有的人想学没处学,有的根本不想学,最后的结果是研究生给本科生干活,老师给学生干活。所以有空的时候,想以这种方式写一点数据处理技术,给自己的未来减负。先从最简单的单X多Y图说起。 实验过程中经常遇到系列样品的表征数据,比如红外光谱、X-射线衍射等等,通常这些仪器测定的步长一致,也即X轴完全相同,这时候可以把系列数据绘制在一个图形中,图形的信息量丰富,也方便数据比较。例如这次本科论文中有一位同学的一组红外数据
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