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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of human CDC25C expressed in E. coli. <br>
- 亚型:
Mouse IgG1
- 形态:
Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide.
- 保存条件:
Store at -20°C
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 适应物种:
H
- 保质期:
1-2年
- 抗原来源:
Purified recombinant fragment of human CDC25C expressed in E. coli. <br>
- 目录编号:
252982
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
WB
- 浓度:
见详细说明信息
- 靶点:
CDC25C (1F12)
- 抗体英文名:
CDC25C (1F12) Antibody
- 抗体名:
CDC25C (1F12) Antibody
- 规格:
0.1 ml
CDC25C (1F12) Antibody产品详细介绍:
| 产品名称: | CDC25C (1F12) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 252982 |
| 规格: | 0.1 ml |
| 产品描述: | Cdc25C (Dual specificity phosphatase Cdc25C) is a dosage-dependent inducer in mitotic control. Cdc25C is a tyrosine protein phosphatase required for progression of the cell cycle. Cdc25C directly dephosphorylates CDK1 and activate its kinase activity. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG1 |
| 免疫原: | Purified recombinant fragment of human CDC25C expressed in E. coli. |
| 适用物种: | H |
| 应用范围: | WB |
| 应用说明: | WB: 1:500-1:2000; |
| 别名: | Cdc25C; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C; CDC25C |
| 形态: | Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide. |
| 保存条件: | Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
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文献和实验基 NP培养基 中胚层诱导培养基 ABC3R培养基 C1F培养基 基础培养基 Knockout DMEM DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 DMEM/F12 Penicillin-Streptomycin
/DMEM:F12 Resuspend in an appropriate volume of 10% FBS/DMEM:F12 and keep on ice Add 50μl cell suspension to 50μl 10% FBS/DMEM:F12 containing appropriate amount of antibody to give the final concentration listed below and incubate for 30min
穹顶状培养,以促进类器官生长。 1.细胞制备 ①组织样本置于含高级DMEM/F12和普里莫星的50mL离心管中,4℃保存直至分离。为确保最佳效果,30mg组织需在24h内解离,以防止细胞死亡和冻融损伤。 ②为每份组织样本制备新鲜消化液:在15mL离心管中加入5mL高级DMEM/F12,再加入5mg/mLII型胶原酶和10μMY27632,混匀后4℃保存备用。 ③将组织转移至10cm培养皿中。用两把手术刀将组织切成约1mm³的小块,用移液管加入5mL消化液。 ④将培养皿中的内容物转移至15
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