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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of human PRDM1 expressed in E. coli.
- 亚型:
Mouse IgG1
- 形态:
Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide.
- 保存条件:
Store at -20°C
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 适应物种:
H, M
- 保质期:
1-2年
- 抗原来源:
Purified recombinant fragment of human PRDM1 expressed in E. coli.
- 目录编号:
252980
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
E, WB
- 浓度:
见详细说明信息
- 靶点:
PRDM1 (5E7)
- 抗体英文名:
PRDM1 (5E7) Antibody
- 抗体名:
PRDM1 (5E7) Antibody
- 规格:
0.1 ml
PRDM1 (5E7) Antibody产品详细介绍:
| 产品名称: | PRDM1 (5E7) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 252980 |
| 规格: | 0.1 ml |
| 产品描述: | Blimp-1 (PRDM1) was initially identified as a zinc finger-containing protein that drives the maturation of B lymphocytes into immunoglobulin-secreting cells. Together with X-box-binding protein 1 (XBP1), Blimp-1 is induced upon terminal differentiation of plasma cells. The transcriptional repressor activity of Blimp-1 has also been found to regulate T cell homeostasis and function, possibly by suppressing the expression of the cytokines IL-2 and interferon-gamma during T cell development. More recent experiments have suggested that Blimp-1 also plays a major role in the formation of primordial germ cells (PGC) in developing mammalian embryos. In these experiments, Blimp-1-deficient mutant mouse embryos form a cluster of PGC-like cells which fail to show the expected migration, proliferation, and repression of homeobox genes that normally accompany specification of primordial germ cells. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG1 |
| 免疫原: | Purified recombinant fragment of human PRDM1 expressed in E. coli. |
| 适用物种: | H, M |
| 应用范围: | E, WB |
| 应用说明: | E: 1:10000; WB: 1:500-1:2000 |
| 别名: | PR domain zinc finger protein 1; BLIMP-1; Beta-interferon gene positive regulatory domain I-binding factor; PR domain-containing protein 1; Positive regulatory domain I-binding factor 1; PRDI-BF1; PRDI-binding factor 1; BLIMP1; PRDM1 |
| 形态: | Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide. |
| 保存条件: | Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
ABBIOTEC产品提供质量保证,提供完善的售后和技术支持,ABBIOTEC产品正品包装:
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文献和实验,其中四种最为重要,分别称为M、N、S、s抗原,它们对应的抗体都与 输血反应有关。M、N、S、s抗原均为糖蛋白,与MN有关的糖蛋白称为血型糖蛋白A(glycophorins A,GPA),与Ss有关的称为血型糖蛋白B(glycophorins B,GPB)。 2.人类血型系统,详见 http://zh.wikipedia.org/zh/%E4%BA%BA%E7%B1%BB%E8%A1%80%E5%9E%8B%E7%B3%BB%E7%BB%9F
质的制备:为了获得足量的染色质,我们的样品准备一定要充足。(IP实验的损耗较大,为保证实验用量,一次准备5×10E7个以上细胞较好) 2、 优化交联固定条件:ChIP实验成功与否,直接取决于染色质完整性、抗原表位质量和抗体特异性(抗体一定买好的)。因此优化交联固定条件,富集丰度更高。 3、 优化染色质片段化条件:要得到理想的ChIP结果,合适长度和浓度的染色质样品至关重要。(推荐的片段化程度为的染色质片段在150-900bp之间)。 4、 免疫沉淀优化:在ChIP抗体富集优化实验中,使用
能的完全人抗体。用此方法可制备针对简单或复杂抗原的单抗,并得到中等亲和力的抗体,但该方法需用高通量筛选。现有一些已进入临床II/III期研究,如Cambridge Antibody Technology(CAT)的D2E7和CAT-152,分别用于治疗风湿性关节炎和青光眼。D2E7是抗TNF-α单抗,现正由Abbott Laboratories开发。从其2002年6月份提供的临床III期数据看,有望于近期进入市场。2、转基因动物制备人源性抗体抗体衍生物:有的研究者正在开发更容易生产和使用的单抗衍生
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