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文献和实验Culture of Primary Human Bronchial Epithelial Cells
The human airway is normally kept sterile despite continual exposure to airborne pathogens. This is achieved by host defense mechanisms that exist to prevent adherence, colonization, and invasion of the airway epithelium. The airway
Primary Mouse Small Intestinal Epithelial Cell Cultures
for optimal growth factor combinations based on genetic evidence of self-renewal regulation, differentiation, and carcinogenesis of intestinal stem cells. Here, we describe methods that we have established for the isolation and culture of primary small
Gene Transfer into Cystic Fibrosis Airway Epithelial Cells
Gene transfer into airway epithelial cells becomes a particularly motivating goal as far as cystic fibrosis (CF) is concerned. As mentioned in Chapter 15 , approx 90% of deaths caused by this devastating disease are the result of infections
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