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- CCL-1
- 2025年11月17日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 细胞类型：
动物细胞
- ATCC Number：
CCL-1
- 组织来源：
肌肉
- 物种来源：
小鼠
- 细胞形态：
fibroblast
- 是否是肿瘤细胞：
否
- 器官来源：
皮下结缔组织
- 运输方式：
干冰
- 生长状态：
贴壁
NCTC clone 929; Areolar Fibroblast; Mouse (Mus Musculus)
NCTC克隆929[L细胞，L-929，L菌株的衍生物]（结缔组织小鼠）是L菌株的第一个克隆，L菌株是首批在连续培养中建立的细胞菌株之一。NCTC克隆929可用于细胞系毒性测试。
General
Specific applications
This cell line can be used for toxicity testing.
This cell line is a suitable transfection host.
这种细胞系可用于毒性测试。
该细胞系是合适的转染宿主。
Characteristics
Growth properties
Adherent
Derivation
The parent L strain was derived from normal subcutaneous areolar and adipose tissue of a 100-day-old male C3H/An mouse.
NCTC clone 929 (Connective tissue, mouse) Clone of strain L was derived in March, 1948. Strain L was one of the first cell strains to be established in continuous culture, and clone 929 was the first cloned strain developed.
Clone 929 was established (by the capillary technique for single cell isolation) from the 95th subculture generation of the parent strain.
Age
100 days
Gender
Male
Strain
C3H/An
Karyotype
modal chromosome number = 66; range = 65 to 68. There were approximately 20 to 30 marker chromosomes present in each metaphase spread. A high percentage of those markers were common to most analyzed cells. A long metacentric chromosome with secondary constriction was noted in 77/100 cells. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Tumorigenic
Yes;
Yes, in immunosuppressed mice
Virus susceptibility
Vesicular stomatitis, Glasgow (Indiana)
Vesicular stomatitis, Orsay (Indiana)
Encephalomyocarditis virus
Comments
This cell line has been tested and found negative for ectromelia virus (mousepox).
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.
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- 作者
- 内容
- 询问日期
文献和实验Curated Citations
Kazazian HH Jr., et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed: 6325324
Fisher EM, et al. Homologous ribosomal protein genes on the human X and Y chromosomes: escape from X inactivation and possible implications for Turner syndrome. Cell 63: 1205-1218, 1990. PubMed: 2124517
Sanford KK, et al. The growth in vitro of single isolated tissue cells. J. Natl. Cancer Inst. 9: 229-246, 1948.
Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111
ASTM International Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices. West Conshohocken, PA: ASTM International;ASTM Standard Test Method F 0813-07.
头一次自己培养L929细胞，手忙脚乱的，把冷冻的细胞直接放进培养基里就结束了。过了60个小时，去显微镜下看，只能看到几个帖壁细胞。仔细想想做实验的步骤，又上网查查帖子，觉得可能是没有在第二天换新培养基的问题，导致冷冻液里的DMSO影响细胞贴壁。不知道培养皿里的细胞还有没有活下去的可能。总之第一次做实验结果不好也不应该轻易放弃。 把培养皿里的上清液转移到离心管里，离心，加入新的培养基重新放到培养箱里培养，原来的培养皿除掉上清液后直接加入新的培养基，因为里面
该词来源于希腊文的 Kλωγ，原义为小枝之集聚，但后来则指由无性繁殖所产生的同基因型的生物群，这是于 1903年由 H． J． Webber最早应用于生物学上的。过去曾译作具有繁殖意思的营养系或含有原语源意思的分支系（群），而现在则多按其原音直接称为克隆。现在该词在个体、细胞和基因等三方面都使用，而且任何一方面似乎都意味着来自同源的犹如复制一样的同一生物群体。（ 1）关于个体：由无性繁殖所增殖的种群均是克隆，这在植物方面，通过体细胞培养，在试管内繁殖、分化可以重新生出完整
在伯内特（F． M． Burnet）关于免疫的无性繁殖系选择学说中，对自身构成物质有发生免疫反应可能的某淋巴系细胞群，称为禁忌细胞株。禁忌细胞株虽依靠对自身的免疫耐受性而制止活动；但可以假定那种抑制机理一失败，那些细胞就对自身发生免疫反应，而出现自身免疫病。
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