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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
默瑞(上海)生物科技有限公司
- 规格:
1000 rxn of 50 µl
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文献和实验Troubleshooting for PCR and multiplex PCR
seaprately using the same PCR program. If one of the primer pairs yields unspecific products, keep the cycling conditions constant and change other parameters as mentioned above (#1 and #2). Mix equimolar amounts of primers and run the multiplex reaction
-crack to help liberate the DNA. Check that the solution actually freezes. Overlay with a drop of mineral oil and incubate at 60°C for 60 minutes, followed by 95°C for 15 minutes. Cool to 4°C. Pipette 22.5 l of PCR master mix
Troubleshooting for PCR and multiplex PCR
Troubleshooting for PCR and multiplex PCR Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles. COMPONENT
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