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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1 年
- 英文名:
2×LAMP Master Mix with Dye
- 供应商:
近岸蛋白
- 规格:
E223-01A 100次/¥1700; E223-01B 500次/¥6800
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文献和实验and in the dark until needed.(Cover tubes with aluminum foil). B.Preparation of Master Mix (Estimated Time 15-30 minutes) NOTE: Make sure you have a water bath heating to 70ºC while mixes are being prepared.It will need to be up to temperature prior to loading
. If you use less than 8.4 ul you will need to replace the rest of that volume with water to make a total of 8.4 ul. 3. To avoid a lot of pippetting when you do PCR you set up a master mix. This mix will contain the sybr green dye, Taq, nucleoties
of a master mix containing water, buffer, dNTPs, primers and Taq DNA Polymerase in a single tube, which can then be aliquoted into individual tubes. MgCl2 and template DNA solutions are then added. This method of setting reactions minimizes the possibility
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