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文献和实验. Cocktail solutions are stored at -20 degrees and are good for approximately 6 months. I. Protease Inhibitor Cocktail The Protease inhibitor cocktail is used as 100X for extract preps and as 1000X for column elution buffers**. Cocktail
before use. Cocktail solutions are stored at -20 degrees and are good for approximately 6 months. I. Protease Inhibitor Cocktail The Protease inhibitor cocktail is used as 100X for extract preps and as 1000X for column elution buffers
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
:chloroform:isoamyl alcohol, UltraPure (25:24:1 v/v/v; Invitrogen) Phosphate-buffered saline (PBS; GIBCO 20012) Protease inhibitor cocktail (Sigma P8340) Proteinase K (10 mg/mL in TE buffer, pH 8.0; Invitrogen) PureLink HQ 96 Mini
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