• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Protease Inhibitors

        互联网

        858

         

         

        <center> <h1> <b><font>Protease Inhibitors</font> </b></h1> </center>

         

        <center> <font>from Alberts' Lab Protocols (Kathy Miller/Chris Field)</font></center>

         

         


        Protease inhibitors are rapidly inactivated in aqueous solutions and should be added to solutions immediately before use. Cocktail solutions are stored at -20 degrees and are good for approximately 6 months.

         

         


        I. Protease Inhibitor Cocktail

        The Protease inhibitor cocktail is used as 100X for extract preps and as 1000X for column elution bufferundefined._Cocktail_is_made_in_100~7_EtOH_and_contains~I~K~Hp~M~2~1~Kp~M~2~1~0 

        inhbitor

         

        <center> <font>Sigma catalog #</font></center>

         

        <center> <font>powder storage</font></center>

         

        <center> <font>concentration in cocktail</font></center>

         

        <center> <font>amt for 25 mls cocktail</font></center>

         

         

         

         

         

        Benzamidine HCL

         

        <center> <font>B6506</font></center>

         

        <center> <font>4 deg C</font></center>

         

        <center> <font>1.6 mg/ml</font></center>

         

        <center> <font>40 mg</font></center>

        Phenanthroline

         

        <center> <font>P1294</font></center>

         

        <center> <font>room temp</font></center>

         

        <center> <font>1 mg/ml</font></center>

         

        <center> <font>25 mg</font></center>

        Aprotiniundefined

         

        <center> <font>A1153</font></center>

         

        <center> <font>4 deg C</font></center>

         

        <center> <font>1 mg/ml</font></center>

         

        <center> <font>25 mg</font></center>

        Pepstatin undefined

         

        <center> <font>P5318</font></center>

         

        <center> <font>4 deg C</font></center>

         

        <center> <font>1 mg/ml</font></center>

         

        <center> <font>25 mg</font></center>

        Leupeptiundefined

         

        <center> <font>L2884</font></center>

         

        <center> <font>-20 deg C</font></center>

         

        <center> <font>1 mg/ml</font></center>

         

        <center> <font>25 mg</font></center>
        ~undefined inhibitors purchased in one-time use amounts

         

        ~undefined_some_components_are_not_completely_soluble_in_100~7_EtOH._Vortex_solution_well_before_use_to_obtain_a_cloudy_suspension_and_add_to_buffer_~F_they_become_soluble_in_aqueous~K~Hp~M~2~1~Kp~M~2~1~0PMSF_is_very_unstable_in_aqueous_solutions._The_100_mM_stock_solution_is_made_in_isopropanol_and_stored_in_the_dark_at_~F20_degrees._Must_be_warmed_~A37_degrees~B_to_get_into_solution._Use_at_1_mM_in_solutions.~K~Hp~M~2~1~Kp~M~2~1~0 

        compiled by Chad Rappleye

        Aroian Lab Protocols

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序