- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-32430c
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken Dihydrofolate reductase,DHFR
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken Dihydrofolate reductase, DHFR ELISA KIT
ELI-32430c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Dihydrofolate reductase, DHFR ELISA KIT is for the quantitative in vitro determination of Chicken DHFR concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Dihydrofolate reductase, DHFR ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken DHFR in the sample, this Chicken Dihydrofolate reductase, DHFR ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken DHFR concentration. The concentration of Chicken DHFR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验二氢叶酸还原酶 dihydrofolate reductase
利用NADPH 还原二氢叶酸产生四氢叶酸的氧化还原酶。 EC1.5.1.3. 。存在于微生物和肝脏中的二氢叶酸还原酶曾有所细致的研究。分子量约为 2 万。似乎也有催化叶酸使之还原成二氢叶酸的作用，所以可将叶酸成为该酶辅酶的活性型的四氢叶酸。在核酸合成旺盛的白血病患者的白血球中含量很多，而其抗癌药氨 [ 基 ] 蝶呤（ 4- 氨基蝶酰 [ 基 ] 谷氨酸）等的作用机理，可能是因为在低浓度（ 10 -8M ）下可抑制此酶而抑制核酸的合成。
Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a Reporter
. In this article, we describe such a method. The method relies on the fact that increasing the expression of dihydrofolate reductase (DHFR) makes Escherichia coli resistant to Trimethoprim (TMP). Proteins fused to DHFR will produce chimeras
Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe
Inactivating a specific protein in vivo can yield important information about its function. One strategy previously developed inSaccharomyces cerevisiae by the Varshavsky group involves fusing a degron, derived from mouse dihydrofolate
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