- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-27991c
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测鸡血清，血浆等样本中目标蛋白的浓度
- 适应物种：
鸡
- 标记物：
Chicken Putative glycerol kinase 5,GK5
- 样本：
鸡的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Chicken Putative glycerol kinase 5, GK5 ELISA KIT
ELI-27991c
FOR LABORATORY RESEARCH USE ONLY. NOT FOR
USE IN DIAGNOSTIC PROCEDURES.
Chicken Putative glycerol kinase 5, GK5 ELISA KIT is for the quantitative in vitro determination of Chicken GK5 concentrations in serum - plasma - tissue homogenates - other biological fluids.
INTENDED USE AND TEST PRINCIPLE
This Chicken Putative glycerol kinase 5, GK5 ELISA KIT is intended Laboratory for Research use only and it is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Chicken GK5 in the sample, this Chicken Putative glycerol kinase 5, GK5 ELISA KIT includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Chicken GK5 concentration. The concentration of Chicken GK5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8 degree. Refer to the expiration date on the label.
MICROTITER PLATE 12x8strips
STANDARD 6x0.3ml vial
SAMPLE DILUENT 6.0ml
ENZYME CONJUGATE 10.0ml
WASH SOLUTION 25ml
SUBSTRATE A 6.0ml
SUBSTRATE B 6.0ml
STOP SOLUTION 6.0ml
Closure plate membrane 2
User manual 1
Sealed bags 1
Note:
1.This package insert must be read in its entirety before using this product.
2.This ELISA Kit is used for lab research only!
"(本产品仅供体外科研用途，不可用于临床诊断!)
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文献和实验cDNA sequencing based on PCR and random shotgun cloning
because once the sequence of a genomic DNA containing cosmid is obtained and putative exons are predicted, the corresponding cDNAs should be sequenced in a timely manner. However, the presently implemented directed cDNA sequencing strategies, i.e. primer walking (17
-89 个氨基酸的N末端调节区的部分p21-activated kinase (PAK-1)相结合。PAK-1的这个区域,被认为是p21偶联区域即P21binding domain (PBD),特异性的与活性形式的Rac1相作用,最终提供了一个优秀的工具以分离细胞样本中的活性Rac1. 细胞被镁盐缓冲溶液（ magnesium lysis buffer)所裂解; 接着裂解液需要采用GST-agarose beads (not provided)进行预清除.在这一步，少量的细胞裂解液能够储存
,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
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