Canine Pepsin B,PGB ELISA试剂盒

Canine Pepsin B, PGB ELISA KIT

Product Name:Canine Pepsin B, PGB ELISA KIT
Packing:96T

Catalog No.:ELI-21572d

Gene Name:Canine Pepsin B, PGB

Detect Range:15.625-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Canine Pepsin B, PGB

Alternative Name:Pepsin B,Pepsin B, PGB
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Pepsin B, PGB ELISA KIT allows for the in vitro quantitative determination of Canine Pepsin B, PGB concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Pepsin B, PGB ELISA Kit (CUSTOM)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

 

Alternative name

Canine PGB ELISA kit ,Canine pepsinogen B ELISA KIT ,Canine PGC ELISA KIT ,Canine PGNB ELISA KIT ,Canine progastricsin ELISA KIT ,Canine pepsinogen C ELISA KIT ,Canine Parapepsin I ELISA KIT

 

Intended use

Canine PGB ELISA kit allows for the in vitro quantitative determination of Canine Pepsin B concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

Canine PGB ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PGB, During the reaction, PGB in the sample or standard competes with a fixed amount of biotin-labeled PGB for sites on a pre-coated Monoclonal antibody specific to PGB. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized product    Min.Order 5kits

Delivery time 4-6month

Order Canine PGB ELISA kit by email:sales@

 

 

has been pre-coated with an Canine Pepsin B, PGB antibody specific to Canine Pepsin B, PGB .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Pepsin B, PGB and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Pepsin B, PGB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Pepsin B, PGB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

  "(本产品仅供体外科研用途，不可用于临床诊断!)