Canine Thromboxane B2 ELISA试剂盒

Canine Thromboxane B2 ELISA Kit

Product Name:Canine Thromboxane B2 ELISA Kit
Packing:96T

Catalog No.:ELA-E0877d

Gene Name:Canine Thromboxane B2

Detect Range:0.313-20ng/ml

Sensitivity:0.188ng/ml

Target Protein Name:Canine Thromboxane B2

Alternative Name:Thromboxane B2,Thromboxane B2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Thromboxane B2 ELISA Kit allows for the in vitro quantitative determination of Canine Thromboxane B2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Thromboxane B2, TXB2 ELISA Kit

96 Tests

Operating instructions

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Thromboxane B2,TXB2

 

Search name

Canine TXB2 ELISA Kit, Canine Thromboxane B2 ELISA Kit, TXB2 ELISA Kit, Thromboxane B2 ELISA Kit

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine Thromboxane B2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thromboxane B2, During the reaction, Thromboxane B2 in the sample or standard competes with a fixed amount of biotin-labeled Thromboxane B2 for sites on a pre-coated Monoclonal antibody specific to Thromboxane B2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Thromboxane B2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

has been pre-coated with an Canine Thromboxane B2 antibody specific to Canine Thromboxane B2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Thromboxane B2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Thromboxane B2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Thromboxane B2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

  "(本产品仅供体外科研用途，不可用于临床诊断!)