- ￥2260
- LSM Bio
- 进口
- PAab09871
- 2025年07月06日
- ELISA, IF, WB, IHC, IP
- E. coli - derived recombinant protein
- Human, Mouse, Rat, Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
catenin(cadherin-associated protein), beta 1, 88kDa
- 亚型：
IgG1
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
monoclonal
- 标记物：
Non-conjugated
- 适应物种：
Human, Mouse, Rat, Pig
- 保质期：
6个月
- 抗原来源：
Mouse
- 目录编号：
Q9BZJ3
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA, IF, WB, IHC, IP
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
catenin(cadherin-associated protein), beta 1, 88kDa
- 抗体英文名：
beta-Catenin antibody
- 抗体名：
beta-Catenin antibody
- 规格：
100μl
beta-Catenin antibody
Product Name beta-Catenin antibody
Catalog No PAab09871
Packing 100ul
Form liquid
Alternative Name b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin
Purification protein A+G purified
Purity 95% as determined by SDS-PAGE
Host Mouse
Isotype IgG1
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Immunogen catenin(cadherin-associated protein), beta 1, 88kDa
Specificity Human, Mouse, Rat, Pig
Tested Application ELISA, IF, WB, IHC, IP
Recommended dilution WB: 1:2000-1:20000; IP: 1:1000-1:5000; IHC: 1:200-1:1000; IF: 1:20-1:500
IHC use Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using PAab09781(B-catenin Antibody) at dilution of 1:200
WB use
A431 cells were subjected to SDS PAGE followed by western blot with PAab09781(B-catenin antibody) at dilution of 1:5000
Protein Information
Gene ID 1499
Uniprot ID P35222
Calculated MW 90 kDa
Research Area Epigenetics, Signal Transduction, Metabolism, Immunology, Cancer, Neuroscience, Stem Cells
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文献和实验familar 小妹困惑，在此请教大师！ 做了几次beta-catenin的表达（western），都没做出来，目的蛋白的分子量是92，可是，做出来的印记总是在下方，明显的不是目的蛋白的位置。我师兄说，做beta-catenin要提核蛋白才能做出来，可我之前也有做出来过的！请教各位有经验的同志，这样的现象是何解？ woxingwosu 不一定要提核蛋白才能出来。b-catenin在核内核外都有分布。应该
lope 用WB检测beta-catenin的活性，应该是一种检测磷酸化水平，一种检测核内水平。 用前一种方法可以省下一个核内参，需要同时检测非磷酸化水平的吗？ 而我看到的文献似乎还是检测核beta-catenin更多，请问前辈们是不是检测核蛋白更容易出结果呢？ ylhuang0502 如检测磷酸化水平，LZ还需要检测总的catenin （磷酸化和非磷酸化都识别）， 将p-beta-catenin
。 如果beta-catenin抗体不好使，或者EGFP太大影响定位的话，还可以构建Flag标签类载体，转染细胞，加入药物，用抗Flag抗体免疫荧光检测，荧光显微镜下观察就好了。 wangjh28 但按照事先的推测，处理了以后会下调该基因的表达 我觉得科学不能有事先的推测的结果，这样容易受思维定势的影响，而忽略了新发现 dongreyuan 我打算用SAB的β-catenin（Ab-37）Antibody，说明
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