木瓜蛋白酶

别名	木瓜酶
CAS	9001-73-4
纯度	98%
单位	瓶

 

木瓜蛋白酶papain属巯基蛋白酶,具有较宽的底物特异性,作用于蛋白质中L-精氨酸、L-赖氨酸、甘氨酸和L-瓜 木瓜蛋白酶氨酸残基羧基参与形成的肽键。

Our liquid papain code PAP is supplied as a suspension in 50mM sodium acetate usually around 1,000 units/ml.  The appearance is a milky viscous suspension. As a suspension it is not homogeneous, the enzyme will appear to settle out if left sitting. So the customer should be sure to invert several times before sampling.  Papain is viscous when refrigerated. You can warm the bottle in your hands (room temperature) to make it easier to handle, invert several times to ensure homogeneity before sampling. Papain is not sterile, it should be 0.22. micron filtered after dilution.  Our papain is routinely used for cell culture.
用途：木瓜蛋白酶在准备细胞培养液（cell culture）的第一步中被用于分离细胞。用酶处理小组织块10分钟后，就可以将连接细胞的细胞外基质打断；然后再用蛋白酶抑制剂来停止反应，防止木瓜蛋白酶进一步裂解细胞本身；最后用Pasteur pipette将组织块打散为单细胞悬浮液。
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