小鼠 EGFR蛋白|EGFR protein|EGFR(Mouse, C-His)(产品说明)
蛋白名称:EGFR蛋白, EGFR protein
蛋白构建:A DNA sequence encoding the extracellular domain of mouse EGFR (Q01279) (Met 1-Ser 647) was fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA.2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind human EGF-Fc (Cat:10605-H01H), The EC50 of human EGF-Fc (Cat:10605-H01H) is 60-90 ng/mL.3. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind mouse EGF-Fc (Cat:50482-M01H), The EC50 of mouse EGF-Fc (Cat:50482-M01H) is 70-100 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Leu 25
蛋白分子量:The secreted recombinant mouse EGFR comprises 634 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, the apparent molecular mass of rmEGFR is approximately 100 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:Q01279
蛋白氨基酸序列:Met1-Ser647
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.