- ￥4980
- Sino Biological
- 10040-HNCH
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
200 µg
蛋白名称：CD30L蛋白, CD30L protein
蛋白构建：A DNA sequence encoding the human CD30L (NP_001235.1) (Gln63-Asp234) was expressed with two additional amino acids (Gly & Pro ) at the N-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE.
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gln 63
蛋白分子量：The recombinant human CD30L consists of 172 amino acids and predicts a molecular mass of 19.6 kDa.
蛋白NP号：NP_001235.1
蛋白氨基酸序列：Gln63-Asp234
蛋白标签：N-cleavage
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L
media additives, DNA and endotoxins have to be removed from the mAb preparation to allow the proteins to be safely applied for human therapy. Moreover, antibody aggregates, clipped and low molecular weight species should also be removed.
Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography
(five or six His residues) placed at either the C- or N-terminus of a recombinant protein can form a stable chelate with immobilized transition metals. This allows fractionation of the target protein to 90–95% purity levels in a single chromatographic step (2 –4 ). Metals
Rationally Designed Ligands for Use in Affinity Chromatography: An Artificial Protein L
. Protein L (PpL), isolated from Peptostreptococcus magnus strains, interacts with the Fab (antigen-binding fragment) portion of Igs, specifically with kappa light chains, and represents an almost universal ligand for the purification of antibodies
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