alpha Lactalbumin / LALBA 蛋白|alpha Lactalbumin / LALBA protein|alpha Lactalbumin / LALBA(Human, His Tag)
- ￥4520
- Sino Biological
- 15631-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
100 µg
蛋白名称：alpha Lactalbumin/LALBA蛋白, alpha Lactalbumin/LALBA protein
蛋白构建：A DNA sequence encoding the human LALBA (NP_002280.1) (Met1-Leu142) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE.
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Lys 20
蛋白分子量：The recombinant human LALBA consists of 134 amino acids and predicts a molecular mass of 15.5 kDa.
蛋白NP号：NP_002280.1
蛋白氨基酸序列：Met1-Leu142
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验LABELING ALPHA-ACTININ WITH IATR
.Dialyze overnight against buffer 5 in the cold room. 11.Clarify at 25,000 rpm,4℃,for 20 min in a 42.2Ti rotor. 12.Determine the concentration of alpha-actinin using Lowry assay.Determine dye/protein molar ratio by diluting conjugate 1:40 (10μl in 400μl
ALPHA FACTOR ARREST AND RELEASE
of approximately 3 x 10�5 M, while bar1 strains require much lower pheromone concentrations (10-8 M). To release cells from alpha factor arrest, spin down cells and wash twice (using a large volume, dependent on the size of the cell pellet anticipated
In situ hybridisation to alpha satellite sequences (chromosome specific)
than this you can put two slides face to face (acting as each other's coverslip) with 30m l of hyb solution. 50m l of Hybridisation solution Solution A 6mls de-ionised formamide 2mls 20 x SSC 2mls H2 O 2g dextran sulphate
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