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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human IL-12 (IL12A & IL12B Heterodimer) Protein
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/10.00 µg/50.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥22680.0 |
|---|---|---|---|
| 规格: | 10.00 µg | 产品价格: | ¥1080.0 |
| 规格: | 50.00 µg | 产品价格: | ¥6470.0 |
| 规格: | 100.00 µg | 产品价格: | ¥3980.0 |
蛋白名称:Human IL-12 (IL12A & IL12B Heterodimer) Protein
蛋白构建:A DNA sequence encoding the human IL12A (P29459-1)(Met1-Ser219) was expressed, constructed the plasmid 1; A DNA sequence encoding the human IL12B (NP_002178.2)(Met1-Ser328) was expressed, constructed the plasmid 2. The two plasmids were co-expressed and the human IL12A&IL12B heterodimer was purified.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.2-2 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Arg23&Ile23
蛋白分子量:The recombinant heterodimer of human IL12A&IL12B comprises 503(197+306) amino acids and has a calculated molecular mass of 57.2(22.5+34.7) KDa. The apparent molecular mass of human IL12 heterodimer is approximately 36 & 42.4 kDa respectively in SDS-PAGE under reducing conditions.
蛋白NP号:P29459-1 & NP_002178.2
蛋白氨基酸序列:Met1-Ser219 & Met1-Ser328
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
Protein Components of the microRNA Pathway and Human Diseases
, Exportin-5 (Exp-5), TAR RNA binding protein (TRBP) and fragile X mental retardation protein (FMRP), are each related, in various ways, to specific genetic diseases.
Mutational Analysis of the Human Protein C Gene
The single gene for protein C is located at position q13–q14 on chromosome 2 (1 ). Two groups have described human genomic clones of protein C isolated from phage l charon libraries using cDNA for human protein C as hybridization probes
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