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- 详细信息
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
5.00 µg/20.00 µg/50.00 µg/500.00 µg
| 规格: | 5.00 µg | 产品价格: | ¥580.0 |
|---|---|---|---|
| 规格: | 20.00 µg | 产品价格: | ¥1980.0 |
| 规格: | 50.00 µg | 产品价格: | ¥2980.0 |
| 规格: | 500.00 µg | 产品价格: | ¥19380.0 |
重组人 IL-12 (IL12A & IL12B 异源二聚体) 蛋白 (His标签)(产品说明)
蛋白名称:Human IL-12 (IL12A & IL12B Heterodimer) Protein (His Tag)
蛋白构建:A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性:Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.04-0.4 ng/mL.
蛋白内毒素:< 5 EU per mg of the protein.
预测N端:Arg 23 & Ile 23
蛋白分子量:The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208+317) amino acids and has a calculated molecular mass of 60.13 (23.99+36.14) kDa.
蛋白NP号:P29459-1 & NP_002178.2
蛋白氨基酸序列:Met1-Ser219 & Met1-Ser328
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称:Human IL-12 (IL12A & IL12B Heterodimer) Protein (His Tag)
蛋白构建:A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性:Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.04-0.4 ng/mL.
蛋白内毒素:< 5 EU per mg of the protein.
预测N端:Arg 23 & Ile 23
蛋白分子量:The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208+317) amino acids and has a calculated molecular mass of 60.13 (23.99+36.14) kDa.
蛋白NP号:P29459-1 & NP_002178.2
蛋白氨基酸序列:Met1-Ser219 & Met1-Ser328
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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重组人 IL-12 (IL12A & IL12B 异源二聚体) 蛋白 (His标签)
¥580 - 19380










