IL-12 蛋白|IL-12 protein|IL-12(Human, His Tag)

人 IL-12蛋白|IL-12 protein|IL-12(Human, C-His)（产品说明）
蛋白名称：IL-12蛋白, IL-12 protein
蛋白构建：A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured in a cell proliferation assay using anti-CD3 antibody activated human peripheral blood mononuclear cell (PBMC). The ED50 for this effect is typically 0.4-2 ng/ml. The specific activity of recombinant human IL-12 is approximately 1.3 x 104 units/μg, which is calibrated against recombinant human IL-12 WHO Standard (NIBSC code: 95/544).
蛋白内毒素：< 0.01 EU per μg of the protein as determined by the LAL method
预测N端：Arg 23 & Ile 23
蛋白分子量：The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa.
蛋白NP号：NP_000873.2 & NP_002178.2
蛋白氨基酸序列：Met1-Ser219 & Met1-Ser328
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.