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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 品系：
人源细胞
- 细胞类型：
人源细胞
- 肿瘤类型：
无
- 供应商：
蒂科生物
- 库存：
5000
- 英文名：
FHs 74 Int
- 生长状态：
良好
- 年限：
永久
- 运输方式：
干冰
- 器官来源：
人
- 是否是肿瘤细胞：
否
- 细胞形态：
贴壁
- 免疫类型：
详询
- 物种来源：
人
- 相关疾病：
无
- 组织来源：
人
- 规格：
T25
*/ 该细胞为ATCC源细胞，由蒂科生物引进 /*
FHs 74 Int人小肠上皮细胞
ATCCâ Number: CCL-241™
Lot Number: 70019552
Name: FHs 74 Int
Description: Small Intestine
Species: Human (Homo sapiens)
Volume/Ampule: Approximately 1 mL
Date Frozen: 09JAN2019
Recovery: A T-25 setup at a seeding density of 1.5 x 104 viable cells/cm2 reaches approximately 15% confluence in 1 day and 90% confluence in 3 days.
Product Format: Cells cryopreserved in the appropriate cryopreservation medium Expiration Date:Not applicable
Storage Conditions: Vapor phase of liquid nitrogen
| Test / Method |
Specification |
Result |
| Ampule passage number |
Report results |
22 |
| Population doubling level (PDL) |
Report results |
Not applicable |
| Total cells/ampule (Cell count using Trypan Blue stain method) |
Report results |
4.4 x 105 total cells/ampule |
| Post-freeze viability (Cell count using Trypan Blue stain method) |
≥ 50.0% |
90.8% |
| Growth properties (Visual observation method) |
Adherent |
Adherent |
| Morphology (Visual observation method) |
Epithelial-like* |
Epithelial-like |
| Test for mycoplasma contamination Hoechst DNA stain (indirect) method Agar culture (direct) method PCR-based assay |
None detected None detected None detected |
None detected None detected None detected |
| Species determination: COI assay (interspecies) |
Human |
Human |
ATCCâ Number: CCL-241™
Lot Number: 70019552
| Species determination: STR analysis (intraspecies) |
Human (Unique DNA Profile) TH01: 7, 9.3 D5S818: 11, 12 D13S317: 11, 12 D7S820: 9, 10 D16S539: 11, 12 CSF1PO: 10, 11 Amelogenin: X vWA: 16, 18 TPOX: 8 |
Human (Unique DNA Profile) TH01: 7, 9.3 D5S818: 11, 12 D13S317: 11, 12 D7S820: 9, 10 D16S539: 11, 12 CSF1PO: 10, 11 Amelogenin: X vWA: 16, 18 TPOX: 8 |
| Sterility test (BacT/ALERT 3D) iAST bottle (aerobic) at 32.5°C iNST bottle (anaerobic) at 32.5°C |
No growth No growth |
No growth No growth |
| Human pathogenic virus testing (PCR-based assay for HIV, HepB, HPV, EBV, and CMV) |
Report results |
HIV – None detected HepB – None detected HPV – None detected EBV – None detected CMV – None detected |
* Epithelial-like: Any adherent cells of a polygonal shape with clear, sharp boundaries between them.
Quality Assurance Specialist; Quality Assurance
ATCC hereby represents and warrants that the material provided under this certificate is pure and has been subjected to the tests and procedures specified and that the results described, along with any other data provided in this certificate, are true and correct to the best of the company’s knowledge and belief. This certificate does not extend to the growth and/or passage of any living organism or cell line beyond what is supplied within the container received from ATCC.
This product is intended to be used for laboratory research use only. It is not intended for use in humans, animals, or for diagnostics. Appropriate Biosafety Level (BSL) practices should always be used with this material. Refer to the Product Information Sheet for instructions on the correct use of this product.
ATCC products may not be resold, modified for resale, used to provide commercial services, or to manufacture commercial products without prior written agreement from ATCC.
© 2017 ATCC. The ATCC trademark and trade name are owned by the American Type Culture Collection.
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文献和实验不断增殖、分化、向上迁移，以补充绒毛顶端脱落的吸收细胞和杯状细胞。绒毛上皮细胞的更新周期为2～4天。一般认为，内分泌细胞和潘氏细胞亦来源于未分化细胞。 2．固有层 在细密的结缔组织中除有大量小肠腺外，还有丰富的游走细胞，如淋巴细胞、浆细胞、巨噬细胞，嗜酸性粒细胞等。绒毛中轴的固有层结缔组织内有1～2条纵行毛细淋巴管，称中央乳糜管（central lacteal），它的起始部为盲端，向下穿过粘膜肌进入粘膜下层形成淋巴管丛。中央乳糜管管腔较大，内皮细胞间隙宽，无基膜，故通透性大。吸收细胞释出的乳糜
不断增殖、分化、向上迁移，以补充绒毛顶端脱落的吸收细胞和杯状细胞。绒毛上皮细胞的更新周期为2～4天。一般认为，内分泌细胞和潘氏细胞亦来源于未分化细胞。 2．固有层 在细密的结缔组织中除有大量小肠腺外，还有丰富的游走细胞，如淋巴细胞、浆细胞、巨噬细胞，嗜酸性粒细胞等。绒毛中轴的固有层结缔组织内有1～2条纵行毛细淋巴管，称中央乳糜管（central lacteal），它的起始部为盲端，向下穿过粘膜肌进入粘膜下层形成淋巴管丛。中央乳糜管管腔较大，内皮细胞间隙宽，无基膜，故通透性大。吸收细胞释出的乳糜
12－18 小肠腺模式图 （4）内分泌细胞（见后述）。 （5）未分化细胞（undifferentiated cell）：位于小肠腺下半部，散在于其它细胞之间。胞体较小，呈柱状，胞质嗜碱性。细胞不断增殖、分化、向上迁移，以补充绒毛顶端脱落的吸收细胞和杯状细胞。绒毛上皮细胞的更新周期为2～4天。一般认为，内分泌细胞和潘氏细胞亦来源于未分化细胞。 2．固有层在细密的结缔组织中除有大量小肠腺外，还有丰富的游走细胞，如淋巴细胞、浆细胞、巨噬细胞，嗜酸性粒细胞等。绒毛中轴的固有
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