- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E10128r
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Cytochrome c oxidase subunit 3,MT-CO3
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Cytochrome c oxidase subunit 3, MT-CO3 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MT-CO3 ,COIII,COXIII,MTCO3,COX3,MTCO3,COIII,cytochrome c oxidase subunit III,cytochrome c oxidase III,Cytochrome c oxidase subunit 3,Cytochrome c oxidase polypeptide III,
Search name
Rat MT-CO3 ELISA KIT ,Rat COIII ELISA KIT ,Rat COXIII ELISA KIT ,Rat MTCO3 ELISA KIT ,Rat COX3 ELISA KIT ,Rat MTCO3 ELISA KIT ,Rat COIII ELISA KIT ,Rat cytochrome c oxidase subunit III ELISA KIT ,Rat cytochrome c oxidase III ELISA KIT ,Rat Cytochrome c oxidase subunit 3 ELISA KIT ,Rat Cytochrome c oxidase polypeptide III ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Cytochrome c oxidase subunit 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome c oxidase subunit 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytochrome c oxidase subunit 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome c oxidase subunit 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cytochrome c oxidase subunit 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验(ubiquinol cytochrome c oxido reductase or bc1 complex), IV (cytochrome c oxidase), and V (ATP synthase). These respiratory complexes are conserved from the yeast Saccharomyces cerevisiae to human with the exception of complex I, which is replaced
Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices
of the NMDA-type of glutamate receptor. More recently, Cytochrome c oxidase has emerged as a novel target for •NO, which competes with O2 for binding to this mitochondrial complex. This reaction establishes •NO not only as a regulator of cellular metabolism
Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases
, all of which are subunits of the respiratory chain complexes. Of the 13 structural genes, 7 encode subunits of complex I (NADH-CoQ oxidoreductase), 1 encodes the cytochrome-b subunit of complex III (CoQ-cytochrome-c oxidoreductase), 3 encode subunits of complex IV
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