Rat Protein S100- A1, S100a1 E

Rat Protein S100- A1, S100A1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

S100A1,S100,S100-alpha; Protein S100-A1,S100A; S-100 protein alpha chain; S-100 protein subunit alpha; S100 alpha; S100 calcium-binding protein A1; S100 protein alpha polypeptide; S100 calcium binding protein A1

Search name

Rat S100A1 ELISA KIT ,Rat S100 ELISA KIT ,Rat S100-alpha ELISA KIT ,Rat Protein S100-A1 ELISA KIT ,Rat S100A ELISA KIT ,Rat S-100 protein alpha chain ELISA KIT ,Rat S-100 protein subunit alpha ELISA KIT ,Rat S100 alpha ELISA KIT ,Rat S100 calcium-binding protein A1 ELISA KIT ,Rat S100 protein alpha polypeptide ELISA KIT ,Rat S100 calcium binding protein A1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Protein S100-A1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein S100-A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Protein S100-A1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein S100-A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein S100-A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.