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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Proteasome subunit beta type-10,Psmb10
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Proteasome subunit beta type- 10, PSMB10 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PSMB10,LMP10; MECL1; beta2i; low molecular mass protein 10; macropain subunit MECl-1; multicatalytic endopeptidase complex subunit MECl-1; proteasome MECl-1; proteasome catalytic subunit 2i; proteasome subunit MECL1; proteasome subunit beta 7i; proteasome subunit beta type-10; proteasome subunit beta-2i; proteasome (prosome, macropain) subunit, beta type, 10
Search name
Rat PSMB10 ELISA KIT ,Rat LMP10 ELISA KIT ,Rat MECL1 ELISA KIT ,Rat beta2i ELISA KIT ,Rat low molecular mass protein 10 ELISA KIT ,Rat macropain subunit MECl-1 ELISA KIT ,Rat multicatalytic endopeptidase complex subunit MECl-1 ELISA KIT ,Rat proteasome MECl-1 ELISA KIT ,Rat proteasome catalytic subunit 2i ELISA KIT ,Rat proteasome subunit MECL1 ELISA KIT ,Rat proteasome subunit beta 7i ELISA KIT ,Rat proteasome subunit beta type-10 ELISA KIT ,Rat proteasome subunit beta-2i ELISA KIT ,Rat proteasome (prosome ELISA KIT ,Rat macropain) subunit beta type 10 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Proteasome subunit beta type- 10, PSMB10 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Proteasome subunit beta type- 10, PSMB10, During the reaction, Rat Proteasome subunit beta type- 10, PSMB10 in the sample or standard competes with a fixed amount of biotin-labeled Rat Proteasome subunit beta type- 10, PSMB10 for sites on a pre-coated Monoclonal antibody specific to Rat Proteasome subunit beta type- 10, PSMB10. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Proteasome subunit beta type- 10, PSMB10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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